Answer and Discussion of Quiz Set: A-005

2.    Which of the following stains would be negative in Whipple’s disease?

A.  PCR for 16S ribosomal RNA

B.  Present with diarrhea and weight loss

C.  Acid fast stain

D.  Brown and Hobbs stain (tissue Gram stain)

E.  GMS stain

      Answer and Discussion: The answer is (C). Tropheryma whipplei is positive for GMS, PAS/D, and GRAM satin. PCR for bacterial 16S ribosomal RNA is becoming standard test. Acid fast test would be negative.